Extended Data Fig. 5: Cryo-EM data processing for apo GABAB.
From: Structural basis of the activation of a metabotropic GABA receptor
a, Single-particle cryo-EM data processing scheme using cryoSPARC. b, Micrograph showing picked particles. c, Representative 2D class averages show distinct secondary structure features. d, Particle angular distribution of the final cryo-EM reconstruction. e, Structural model shown in cartoon representation and coloured by the B-factors. f, Density map coloured by local resolution, showing higher resolution at the interface of GB1 and GB2. g, Gold-standard FSC curves from cryoSPARC. h, Map-to-model FSC curve between each refined model and the corresponding sharpened electron potential maps. i, GB1 and GB2 interact through LB1s and intracellular tips of TM3 and TM5, with a total interface area of 963 Å2. Residues involved in dimerization contacts are coloured in purple.
