Extended Data Fig. 1: Absence of coelomocytes causes LBP-2 to accumulate in pseudocoelom together with secreted GFP.
From: Extracellular proteostasis prevents aggregation during pathogenic attack
a, LBP-2::tagRFP expression pattern in body-wall muscles (day 2, n = 14 worms). b, c, Secreted LBP-2::tagRFP and secreted GFP colocalize in day 2 animals with coelomocytes (n = 16 worms) (b) and accumulate in animals without coelomocytes (n = 6) (c). Asterisk indicates pharyngeal GFP reporter in animal without coelomocytes. b, c, Secreted GFP exposure, 5 times shorter in c versus b, secreted LBP-2::tagRFP, identical exposure. d, LBP-2::tagRFP puncta in tail region (day 2, n = 15 worms; day 8, n = 14). Maximum projection. Scale bar, 20 μm. e, Quantification of LBP-2::tagRFP aggregation with age in the tail (n = 2 independent experiments). P values determined by two-sided Fisher’s exact test (day 8) and chi-square test (day 12). f, LBP-2 aggregates are separate from neurons (n = 27 worms). Scale bar, 20 μm. Single plane. g, Total protein stain of blot in Fig. 1j (n = 2 independent experiments) with fold changes quantified per fraction relative to levels in day 2. For blot source image, see Supplementary Fig. 1.
