Extended Data Fig. 3: ECRs regulate LYS-7 aggregation.
From: Extracellular proteostasis prevents aggregation during pathogenic attack
a, Quantification of animals without GFP-labelled coelomocytes in transgenic animals expressing secreted GFP (GS1912) treated with RNAi targeting 13 top candidates (n = 2 independent experiments). Ctrl (−) denotes empty vector; ctrl (+) denotes dyn-1 RNAi. b, LYS-7::tagRFP in young whole animal. Arrowheads indicate localization in coelomocytes, and asterisk indicates localization in anterior intestinal cells (top panel, n = 10 worms). LYS-7::tagRFP diffuse localization in head region of young animal (bottom left panel, n = 19) and puncta localized in head region of aged animal (bottom right panel, n = 14). Laser intensity 15%, maximum projection. Scale bar, 20 μm. c, Quantification of LYS-7::tagRFP aggregation with age (n = 2 independent experiments). d, Effect on LYS-7::tagRFP aggregation at day 6, 25 °C, with RNAi targeting top 13 candidates (n = 4 independent experiments). e, LYS-7 aggregation is reduced by CLEC-1 overexpression quantified at day 6 (n = 2 independent experiments). Ctrl indicates CLEC-1 non-overexpressing animals. P values determined by two-sided Fisher’s exact test with Benjamini–Hochberg correction.
