Extended Data Fig. 6: Impairing extracellular proteostasis accelerates intoxication-related mortality.
From: Extracellular proteostasis prevents aggregation during pathogenic attack
a, Expression level of LBP-2::tagRFP is not reduced after 3 h exposure to 100% Cry5B. Data are mean ± s.e.m. of n = 4 biologically independent samples. b, Quantification of LBP-2::tagRFP aggregation upon exposure to Microbacterium nematophilum and Bacillus atrophaeus at day 6 (n = 2 independent experiments). c, Expression level of four selected ECR candidates in unchallenged conditions with control pQE9 empty vector in kgb-1(km21) mutant versus wild-type background. d, Expression levels of eight selected ECRs with vhp-1 versus control RNAi. Data are mean ± s.e.m. of n = 3 (c) and n = 4 (d) biologically independent samples. e, Survival analysis of LBP-2::tagRFP transgenics on jnk-1(gk7) versus wild-type background subjected to 50% Cry5B (n = 2 independent experiments). f, Survival analysis of LBP-2::tagRFP transgenics subjected to 25% Cry5B with RNAi targeting selected ECRs (n = 2 independent experiments). g, Survival analysis of secreted GFP transgenics with versus without coelomocytes, subjected to 50% Cry5B (n = 4 independent experiments). h, Survival analysis of LBP-2::tagRFP transgenics versus N2 wild-type subjected to 50% Cry5B (n = 1 independent experiment). i, Survival analysis of LBP-2::tagRFP transgenics with and without secreted GFP overexpression subjected to 50% Cry5B (n = 2 biologically independent samples). j, Increased LBP-2::tagRFP aggregation at day 4 of adulthood during exposure to 25% Cry5B and treatment with RNAi targeting selected ECRs compared to empty vector (n = 2 independent experiments). P values determined by two-sided unpaired t-test with Welch’s correction (a, c, d), chi-square test (b) and two-sided Fisher’s exact test with Benjamini–Hochberg correction (j), and log-rank test with Bonferroni correction (e–i). For detailed values see Extended Data Tables 2, 3.
