Extended Data Fig. 11: Restoration of complex II by ectopic expression of SDHA cDNA rescues tumour growth.
From: Mitochondrial ubiquinol oxidation is necessary for tumour growth
a, Western blot analysis of SDHA protein levels in 143B-CYTB-Δ-NT, 143B-CYTB-Δ-SDHA_KO2-AOX-RFP and 143B-CYTB-Δ-SDHA_KO2-AOX-cDNA SDHA cells. Data representative of three independent experiments. b, Complex II-driven OCR of permeabilized 143B-CYTB-Δ-SDHA_KO2-AOX-RFP and 143B-CYTB-Δ-SDHA_KO2-AOX-cDNA SDHA cells. Succinate and ADP were provided as substrates. Piericidin A (1 μM) and antimycin A (1 μM) were used to inhibit complex I and III respectively. SHAM (2 mM) was used to inhibit AOX activity (n = 4 biologically independent experiments). c, 143B-CYTB-Δ-SDHA_KO2-AOX-RFP and 143B-CYTB-Δ-SDHA_KO2-AOX-cDNA SDHA cells were grown in the presence or absence of methyl pyruvate and cell number was assessed after 72 h (n = 5 biologically independent experiments). d, e, Average tumour volume (d) and tumour mass (e) of xenografts from 143B-CYTB-Δ-SDHA_KO2-AOX-RFP and 143B-CYTB-Δ-SDHA_KO2-AOX-cDNA SDHA cells (n = 8 mice per group from two independent cohorts). Data are mean ± s.e.m. (b–e). *P < 0.05, **P < 0.01, two-tailed t-tests (e) or two-way ANOVA (c, d) with a Bonferroni test for multiple comparisons (exact P values are in the Source Data). For gel source data, see Supplementary Fig. 6.
