Extended Data Fig. 8: Additional treatments to reduce growth rate.
From: Temperature-dependent growth contributes to long-term cold sensing
a, Inhibition of growth by applying Kan (200 μg/l), AVG (1 μM or 10 μM), ABA (1 mM), 24D (1 μM), IAA (10 μM), Brz (1 μM or 10 μM), ACC (1 μM, 10 μM or 100 μM), HU (10 mM or 20 mM) and PAC (2 μM, 20 μM or 100 μM). Control indicates no treatment. Seedlings grown for around 6 days were transferred to new medium supplemented with the indicated chemicals for 2 days in the warm (20 °C). Two independent experiments showed similar results. Scale bar, 0.5 cm. b, Imaging of the fluorescence signal of GFP-NTL8 in the root tip of plants from a after treatments for 2 days in the warm (20 °C). Scale bar, 100 μm. c, Quantification of the fluorescence intensity averaged over multiple roots. Two independent experiments were combined; roots were imaged per treatment (treatment order as in c from left to right): 16 roots total (6 excluded), 25 (1 excluded), 13 (0 excluded), 55 (3 excluded), 13 (0 excluded), 18 (0 excluded), 19 (0 excluded), 20 (7 excluded), 30 (3 excluded), 52 (0 excluded), 19 (0 excluded), 40 (0 excluded), 25 (0 excluded), 43 (0 excluded), 30 (1 excluded), 42 (0 excluded) and 51 (1 excluded). Error bars are s.e.m. We observed higher NTL8 in all treatments that inhibited growth without killing the plants. Seedlings treated with 10 mM or 20 mM HU for 2 days were almost dead. The 1 μM AVG treatment did not increase NTL8 levels, which is expected as growth was not slowed in that case. ACC treatments showed subtle effects, possibly due to indirect effects.
