Extended Data Fig. 2: Identification of Gad2+ cell types.
From: Distinct subnetworks of the thalamic reticular nucleus
a, t-SNE embedding of 1,687 single nuclei as in Fig. 1a. Single nuclei are coloured by dissection sources as indicated by the colour bar on the right. Different batches of dissections from the external part of globus pallidus (GPE) are coloured differently. n = 1,687 nuclei. b, t-SNE embedding of nuclei showing expression levels (pseudo-colour) of marker genes for each cluster. The three small clusters exhibiting markedly less Pvalb expression, but enriched with a combination of markers including Gabra1, Fxyd6, Tac1. n = 868 nuclei. c, ISH image in sagittal view of selected markers shown in b. Images obtained from Allen Brain Atlas (https://portal.brain-map.org/). Dashed lines indicate boundaries of TRN and the neighbouring GABAergic nuclei. GP: globus pallidus; ZI: zona incerta; BNST: bed nucleus of the stria terminalis.
