Extended Data Fig. 7: Imaging of LILRB1 and perforin in an immunological synapse model of NK cells.
From: Structural basis for RIFIN-mediated activation of LILRB1 in malaria
a, PfRH5 and anti-PfRH5 R5.016 antibody (αRH5), together with ICAM-1, trigger contact formation of NK cells and mobilization of perforin (yellow) to the synapse. Representative images are shown from one of two independent experiments with similar results. b, A calibration curve for the density of pMHC, RIFIN(C223S) (RIFIN) and RIFIN(C223S/G234R) (G234R) on SLBs. c. Additional images of LILRB1 localization (green) in NK cells in the contact area on SLBs coated with RIFIN(C223S), RIFIN(C223S/G234R) or MHC class I (pMHC) (magenta). d, Additional images of perforin mobilization (yellow) in NK cells on SLBs coated with RIFIN(C223S), RIFIN(C223S/G234R) or MHC class I (pMHC) (magenta). Scale bars, 10 μm. Three independent experiments were carried out with cells from different donors with similar results. The raw MFI values of these experiments are shown in Extended Data Fig. 8a–c.
