Extended Data Fig. 3: Analysis of the effect of RIFIN on signalling in a T-cell-based GFP-reporter system. | Nature

Extended Data Fig. 3: Analysis of the effect of RIFIN on signalling in a T-cell-based GFP-reporter system.

From: Structural basis for RIFIN-mediated activation of LILRB1 in malaria

Extended Data Fig. 3: Analysis of the effect of RIFIN on signalling in a T-cell-based GFP-reporter system.

a, The NFAT–GFP-based reporter system, which expresses GFP in response to LILRB1-mediated signalling, was used to assess the effect of RIFIN. Fluorescent signalling of the LILRB1-reporter cells and control reporter cells were assessed in triplicate in the presence of the immobilized variable domain of LILRB1-binding RIFIN (PF3D7_1254800; RIFIN(C223S)), RIFIN(C223S/G234R) and a RIFIN that does not bind to LILRB1 (PF3D7_1254200). b, Flow cytometry images used to derive a.

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