Extended Data Fig. 2: Hypoxia activates Na+/Ca2+ exchange through the NCLX.
From: Na+ controls hypoxic signalling by the mitochondrial respiratory chain
Cytosolic Ca2+ (Ca2+i) or Na+ (Na+i) were measured by live imaging fluorescence microscopy with Fluo-4 AM or CoroNa Green AM, respectively, in normoxia or acute hypoxia (2% O2). a–d, BAECs not treated (No treat) or transfected with siSCR or siNCLX. e–h, BAECs treated or not with the NCLX inhibitor CGP-37157 (10 μM). a, c, e, g, time-course traces; b, d, f, h, slopes. All data are represented as mean ± s.e.m. of n = 3 independent experiments. Two-tailed Student’s t-test (b, d, f, h): * P < 0.05, ** P < 0.01, *** P < 0.001, n.s. not significant.
