Extended Data Fig. 4: NCLX activation in acute hypoxia depends on mitochondrial CI.
From: Na+ controls hypoxic signalling by the mitochondrial respiratory chain
a, b, Assessment of interference of subunits of CI (NDUFS4) or CIII (RISP) by western blot in whole-cell extracts from BAECs. Representative images of two independent experiments. c, d, Cytosolic Ca2+ (Ca2+i; c) or Na+ (Na+i; d) measured by live imaging confocal microscopy with Fluo-4 AM or CoroNa Green AM, respectively, in normoxia or acute hypoxia (1% O2); time-course traces and slopes, n = 4 (c), n = 3 (d). e, f, Effect of OXPHOS inhibitors on cytosolic Ca2+ (e; n = 3) or Na+ (f; n = 6) measured as in c and d. All data are represented as mean ± s.e.m. Two-tailed Student’s t-test or Mann–Whitney U test (c; siSCR). n.s. not significant, * P < 0.05, **P < 0.01. For gel source data, see Supplementary Fig. 1.
