Extended Data Fig. 1: Loss of social recognition in Nlgn3KO mice.
From: Rescue of oxytocin response and social behaviour in a mouse model of autism
a, b, Mean social interaction time and data for individual mice in the social habituation/recognition test plotted for wild-type (n = 11) (a) and Nlgn3KO (b) mice (n = 12). c, d, Mean social interaction time and data for individual mice plotted for DAT-cre control mice (n = 10) (c) and DAT-cre::Nlgn3KO mice (n = 11) (d). e, Example for validation of targeted gene knockdown (n = 8 mice) from AAV2-DIO-miRNlgn3-GFP viruses (green) in TH-positive cells (red) in the VTA of DAT-cre mice. f, Quantification of percentage of TH-positive cells in VTA and SNc of DAT-cre mice that express GFP from the AAV2-DIO-miR-GFP vector (n = 8). g, h, Mean social interaction time and data for individual mice plotted for control mice (g, VTA::DA-miR, n = 10) and VTA DA-specific Nlgn3 loss-of-function (h, VTA::DA-NL3, n = 8) in the social habituation/recognition test. g, Mean social interaction time and data for individual mice plotted for mice treated with vehicle (n = 12) (i) and OXTR-A (n = 11) (j). All error bars are s.e.m. Repeated-measures one-way ANOVA followed by Bonferroni’s post hoc test for planned multiple comparison (a–c, g–j) or Friedman test followed by Dunn’s post hoc test for planned multiple comparison (d). See Supplementary information for additional statistics.
