Extended Data Fig. 5: Pharmacological profile of novel MNK1/2 inhibitor ETC-168.
From: Rescue of oxytocin response and social behaviour in a mouse model of autism
a, b, Quantification of p-ERK1/2 (a) and p-eIF4G (b) levels compared to non-phosphorylated protein in cortical neurons at DIV14 treated with ETC-168. n = 8 replicates from 3 independent experiments. c, d, Quantification of eIF4E (c), ERK1/2 (d) and eIF4G (e) levels normalized to calnexin in cortical neurons at DIV14 treated with ETC-168. P values determined by one-way ANOVA. n = 8 replicates from 3 independent experiments. f, Representative western blot of cerebellar lysate from wild-type mice treated with vehicle or ETC-168 and quantification of p-eIF4E levels compared to eIF4E. n = vehicle: 4; 1 mg kg−1: 3; 5 mg kg−1: 6. g, Representative western blot of VTA lysate from wild-type and Nlgn3KO mice (n = 7 mice per genotype). h, Quantification of p-eIF4E compared with eIF4E levels in VTA of wild-type mice and Nlgn3KO mice. n = 7 mice per genotype. i, Normalized p-eIF4E AlphaLisa counts from wild-type and Nlgn3KO VTA lysate. n = 7 mice per genotype. j, k, Representative western blot (j) and quantification (k) of p-MNK1 and MNK1 levels in VTA lysate from wild-type mice and Nlgn3KO mice. n = 7 mice per genotype. l, Normalized p-eIF4E AlphaLisa counts from VTA from wild-type treated with 5 mg kg−1 ETC-168 for 24 h +2 h. n = 5 mice per genotype. All error bars are s.e.m. P values determined by one-way ANOVA (a, c–f), Kruskal–Wallis test (b), unpaired two-sided t-test (h, k), or two-sided Mann–Whitney test (i, l). See Supplementary Information for additional statistics.
