Extended Data Fig. 6: Rett syndrome mutations disrupt MeCP2 condensate formation.
From: MeCP2 links heterochromatin condensates and neurodevelopmental disease
a, Droplet areas for experiments in Fig. 3b. Fields per condition n = 15. b, Droplet areas for experiments in Fig. 3d. Fields per condition n = 15. c, Droplet areas for experiments in Fig. 3f. Fields per condition n = 15. d, Droplet experiments examining effects of Rett syndrome missense mutations that disrupt IDR-2 on MeCP2 droplet formation. Wild-type MeCP2–GFP and Rett syndrome IDR-2 mutants (P225R and P322L) at indicated concentrations were mixed with 20 nM methylated DNA in droplet formation buffers with 100 mM NaCl. e, MeCP2–GFP condensed fraction as a function of MeCP2–GFP concentration for experiments in d. Data are mean ± s.d. Fields per condition n = 15. f, Droplet areas for experiments in d. Fields per condition n = 15.
