Extended Data Fig. 7: TBLR1 partitioning into MeCP2 droplets is disrupted by Rett syndrome mutation R306C.
From: MeCP2 links heterochromatin condensates and neurodevelopmental disease
a, Droplet experiments examining ability of wild-type MeCP2 and R306C mutant condensates to enrich TBLR1-CTD. Wild-type MeCP2–GFP or R306C mutant at 6 μM was mixed with TBLR1-CTD-mCherry at 10 μM in droplet formation buffers with 125 mM NaCl and 10% PEG-8000. b, TBLR1-CTD-mCherry partition ratios in MeCP2–GFP wild-type and R306C mutant droplets for experiments in a. Fields per condition n = 15. c, Droplet experiments examining ability of wild-type MeCP2 and R306C mutant condensates to enrich TBLR1-CTD. Wild-type MeCP2–GFP or R306C mutant at 10 μM was mixed with TBLR1-CTD-mCherry at 4 μM in droplet formation buffers with 125 mM NaCl. d, TBLR1-CTD-mCherry partition ratios in wild-type MeCP2–GFP and R306C mutant droplets for experiments in c. Fields per condition n = 12.
