Extended Data Fig. 12: Immune evasive wHILOs by enhanced endogenous PD-L1 expression.
From: Immune-evasive human islet-like organoids ameliorate diabetes
a, Schematic showing multi-low dose streptozotocin treatment (MLD-STZ, 50 mg/kg/day for 5 days) of Hu-PBMC-NSG mice to make immune competent diabetic model. MPS induced PD-L1 expressing wHILOs (n = 500) were transplanted under kidney capsule. b, Random-fed blood glucose levels in STZ-induced diabetic Hu-PBMC-NSG mice after transplantation of wHILOs with or without MPS (n = 6). Data for wHILOs (−) from Fig. 3c was used since those experiments were performed in parallel. c, Flow cytometry analyses of insulin-expressing and human immune (CD45+) cells recovered from kidney capsule grafts 27 days after transplantation of wHILOs with or without MPS. Error bars represent ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001, one-tailed, Student’s paired t-test. Data were compiled from b or representative of 2 independent experiments.
