Extended Data Fig. 11: GEF assay for different small GTPases with C9orf72–SMCR8–WDR41.
From: Structure of the C9orf72 ARF GAP complex that is haploinsufficient in ALS and FTD
a, SDS–PAGE of C9orf72–SMCR8–WDR41 complex and GTPase proteins used in the experiments. b, GEF assay with mantGDP-reloaded RAB1A, RAB5A, RAB7A, RAB8A and RAB39B proteins in the absence and addition of C9orf72–SMCR8–WDR41 complex, as indicated. RAB5A treated with RABEX5 was used as a positive-control reaction. Data were baseline-subtracted and normalized to the signal immediately after GTP addition, which also is the 0-s time point in the plots. Plots are mean ± s.d. of each technical triplicate experiment. All experiments were carried out at least twice independently with similar results (a, b). c, Structural alignment of C9orf72–SMCR8–WDR41 with DENND1B–RAB35 (PDB 3TW8).
