Extended Data Fig. 8: Generation of uvrA-deficient L. reuteri.

a, Schematic of the genomic structure of the uvrA gene of L. reuteri (L. reuteri wild type), the targeting vector and the resultant mutant (L. reuteri ΔuvrA) generated by homologous recombination containing the erythromycin-resistance gene (ermAM). The NdeI and SpeI restriction sites are indicated by NI and SI, respectively. b, PCR analysis of genomic DNA from L. reuteri and L. reuteri ΔuvrA using uvrA- and ermAM-specific primers. c, Southern blot analysis of NdeI- and SpeI-digested genomic DNA from L. reuteri and L. reuteri ΔuvrA. Probes A and B, depicted in a, were used to detect genomic fragments present in both strains and only in L. reuteri ΔuvrA, respectively. The experiments were independently repeated two times with similar results (b, c). For gel and blot source data, see Supplementary Fig. 1.