Extended Data Fig. 3: Modulation of TH17-cell function by SAA.
From: Gut microorganisms act together to exacerbate inflammation in spinal cords
a, Splenocytes from germ-free mice inoculated with L. reuteri or OTU0002 were cultured in the presence or absence of MOG35–55 for three days. The cytokine concentrations in the supernatants were measured by ELISA (n = 5 mice). b, Percentage and absolute numbers of TH17 cells in the spleen (n = 5 mice). c, f, mRNA expression of the indicated genes in tissues of the small intestine (n = 5 mice). d, e, Splenocytes from specific-pathogen-free EAE mice were restimulated with MOG35–55 in the presence or absence of SAA. d, After culturing for two days, CD4+ cells were enriched by magnetic beads and the mRNA expression of the indicated genes was quantified by qPCR (n = 4 mice). e, The cytokine concentrations in the supernatants were measured by ELISA (n = 4 mice). g, Representative FACS plots (gated on CD3+ and CD4+ cells), percentage and absolute numbers of TH17 cells in the small-intestinal lamina propria of naive mice (germ-free, n = 6 mice; L. reuteri and OTU0002, n = 5 mice). Data are mean ± s.d. ***P < 0.001, **P < 0.01, *P < 0.05. Two-way ANOVA with Bonferroni’s test (a), one-way ANOVA with Tukey’s test (b–g) or Kruskal–Wallis with Dunn’s test (b, number of IL-17A+CD4+ T cells; c, Saa1, Saa2 and Il12b; d, Rorc and Il17a; f, Csf2; g, number of IL-17A+CD4+ T cells). Exact P values are in Source Data.
