Extended Data Fig. 3: Apical keratin localization requires desmosome protein components.
From: Keratins are asymmetrically inherited fate determinants in the mammalian embryo
a–c, Immunofluorescence of endogenous plakoglobin (a), plakophilin (b), and desmoglein2 (c) before and after apical domain formation. Apical accumulation of all three desmosome components is observed after apical domain formation. d, Live imaging of an embryo expressing desmoglein2-Emerald, RFP-utrophin and H2B-RFP recapitulates the endogenous desmoglein2 expression, both before and after apical domain formation. e, Time series of embryo expressing desmoglein2-Emerald, RFP-utrophin and H2B-RFP. Desmoglein2-Emerald accumulates with the apical domain (labelled by RFP-utrophin) during interphase. When the cell enters mitosis, desmoglein2-Emerald disassembles from the apical surface together with the apical domain. White arrows indicate two different mitotic events within the same embryo. f, Live embryo expressing desmoglein2-Ruby and K18-Emerald shows the enrichment of keratin filaments at the site of apical desmosome accumulation. g, Embryos injected with siRNAs against desmosome components do not accumulate desmoglein2 apically with the apical domain. h, Desmosome knockdown causes a more homogenous distribution of keratin filaments, as measured by a polarization index. **P = 0.01; unpaired, two-tailed Mann–Whitney U-test. Data are from three independent experiments. Scale bars, 10 μm.
