Extended Data Fig. 8: Interaction Nup159-Atg8 upon starvation.
From: In-cell architecture of the nuclear pore and snapshots of its turnover
a, Fluorescence intensity analysis of nup120Δ, Nup170-Mars, Nup159-Atg8-split Venus nuclei spots before and after 5.5 h of starvation. The ratio Mars/Venus shows the significant increase in Venus signal as compared to Mars signal in this starved strain. Three biological replicates and n = 100 spots per replicate were measured (P < 0.00001, Mann–Whitney, two-sided; box centres represent the mean and error bars the s.d.). b, Tomographic slice (plastic section) overlaid with wide-field fluorescent image obtained by on section-CLEM37,45 of the strain in a upon ~6 h of starvation. n = 36 correlated tomograms (technical replicates) were acquired and 24 (75%) had similar results. 8 spots come from uranyl autofluorescence in the cytoplasm. Venus signal is shown in yellow. Nup170-Mars is shown in red. The cyan dashed rectangle indicates the area zoomed into the two right panels showing tomographic slices at two different Z-heights (N marks the nucleus, scale bar: 200 nm). See also Supplementary Videos 9, 10.
