Extended Data Fig. 1: In-cell structure of the S. cerevisiae NPC vs detergent-extracted NPC (EMD-7321).
From: In-cell architecture of the nuclear pore and snapshots of its turnover
a, Gold standard FSCs of in-cell cryo-EM map of the S. cerevisiae NPC. All the curves (nuclear ring NR, cytoplasmic ring CR, inner ring IR) intersect the 0.143 criterium at around 25 Å resolution. b, Local resolution analysis59 with colour-coded bar. c, In-cell cryo-EM map (grey, average of n = 250 NPCs) in comparison to cryo-EM map of detergent-extracted S. cerevisiae NPCs (blue, EMD-7321 at the suggested contour level) show significant differences in diameter and in interpretable features. The nuclear membranes and the Y-complexes are clearly discerned in the in-cell cryo-EM map, in contrast to EMD-7321. d, Tomographic slices through the maps shown in c at the level of the cytoplasmic (CR) and inner rings (IR) and an individual spoke (Sp). Lines in c indicate slicing position shown in d. Arrowheads indicate blurred features in the outer rings. Scale bar: 50 nm.
