Extended Data Fig. 5: Ab initio models for cryo-EM of LRRK2^RCKW dimers and cryo-EM analysis of WD40- and COR-mediated dimers of LRRK2^RCKW in the presence of the inhibitor MLi-2.

a, An initial dataset was collected from a sample of LRRK2^RCKW incubated in the presence of the kinase inhibitor MLi-2 and dimers were selected. b, Representative two-dimensional class averages used for ab initio model building. c, Ab initio models with the structure of LRRK2^RCKW docked in. d, Volumes generated form the molecular models in b, filtered to 30 Å resolution. e, Projections of the volumes in d shown in the same order as their corresponding 2D class averages in b. f, Data processing strategy for obtaining cryo-EM structures of WD40- and COR-mediated dimers of LRRK2^RCKW in the presence of the inhibitor MLi-2. The models used during this processing (Methods) are those shown in d along with an additional linear trimer (Methods) used for particle sorting.