Extended Data Fig. 8: Inhibition of motor motility by wild-type and I2020T mutant LRRK2^RCKW.

a, Example kymographs showing that increasing concentrations of LRRK2^RCKW reduce kinesin runs. b, Example kymographs showing that 25 nM LRRK2^RCKW reduces dynein runs. c, Representative kymographs of kinesin motility in the presence or absence of wild-type and I2020T mutant LRRK2^RCKW. d, The percentage of motile kinesin events per microtubule in the absence of LRRK2 or in the presence of 25 nM wild-type or I2020T mutant LRRK2^RCKW. Data are mean ± s.d. (n = 12 microtubules per condition quantified from two independent experiments). There is a significant difference between 0 nM and both 25 nM RCKW conditions (P < 0.0001), but no significant (ns) difference between the inhibitory effects of wild-type LRRK2^RCKW versus I2020T mutant LRRK2^RCKW as calculated using the Kruskal–Wallis test with Dunn’s posthoc for multiple comparisons (compared to no LRRK2^RCKW).