Extended Data Fig. 6: Localization of viral genomic DNA and histones in HCMV-infected cells.
From: Cytoplasmic control of intranuclear polarity by human cytomegalovirus
a, Representative images of early and mature replication compartments containing viral DNA in HCMV-infected cells, versus uninfected (mock) cells, detected using FISH. DNA-immunoFISH was used to detect the viral immediate early transcription factor, IE1/2 in conjunction with viral DNA. IE1/2 is present in cells containing early, individual replication compartments. As infection progresses, IE1/2 abundance increases and DNA-containing replication compartments amplify and coalesce. b, Representative examples of the relative spatial distribution and fluorescence intensity of histone H3K9me3 foci and viral genomic DNA (gDNA) in HCMV-infected cells at 72 and 96 h.p.i. Note that the bulk of histone H3K9me3 foci are spatially polarized towards the assembly compartment and segregated away from viral gDNA. For all experiments, data shown is representative of 3 independent biological replicates.
