Extended Data Fig. 8: Capacity of mini-gut tubes to regenerate after radiation-induced damage.
From: Homeostatic mini-intestines through scaffold-guided organoid morphogenesis
a, Mini-gut tubes fail to regenerate and rapidly deteriorate upon exposure to 8 Gy radiation dose. Overlaid bright-field and LGR5–eGFP fluorescence time-course images of epithelial damage in mini-gut tubes induced by exposure to 8 Gy radiation dose are shown. Scale bars, 60 μm. b, Rapid replenishment of LGR5–eGFP+ stem cells initially eliminated by exposure to 2 Gy radiation dose. A graph showing normalized fluorescence intensity of eGFP measured in the crypts before and after exposure to 2 Gy and 8 Gy radiation dose. Mean ± s.d. for n = 4 samples. c, Time-course of mini-gut tubes regeneration upon 2 Gy radiation dose-induced damage shown in bright-field and LGR5–eGFP fluorescence. EDF of bright-field images, calculated for a z-stack of 80 μm; fluorescence confocal images correspond to a maximal intensity projection of a z-stack of around 60 μm. Scale bars, 60 μm. All data are representative of at least two independent experiments with n = 3 replicates per each condition.
