Extended Data Fig. 8: Substrate scopes for BACED and pySOOF.
From: Light-driven post-translational installation of reactive protein side chains
a, A comprehensive list of all side chains installed into proteins with the BACED reaction manifold. General conditions: protein 1 mg ml−1; 50 W, 450 nm light; 4 °C to RT; 100–1,500 equiv. BACED precursor reagent; 10 equiv. Cat1 or Cat2; 100 equiv. catechol; <6 ppm O2; pH 6.0 buffer (500 mM NH4OAc or PBS ± 3 M GdnHCl). b, A comprehensive list of all side chains installed into proteins with the pySOOF reaction manifold. General conditions: protein 1 mg ml−1; 50 W, 450 nm light; RT; 2–5 equiv. pySOOF precursor reagent; 0.4–4 equiv. Cat1; 50–100 equiv. FeSO4; <6 ppm O2; pH 6.0 buffer (500 mM NH4OAc or various other buffers).
