Extended Data Fig. 7: SARS-CoV-2 infection of Calu3 cells.
a, Calu3 cells were either left uninfected, infected with SARS-CoV-2, or infected with SARS-CoV-2 in the presence of trypsin. 12 hpi the cells were fixed and stained with antisera against SARS-CoV-2 (green) and DAPI (blue). The experiment was performed once and representative microscopy images are presented. Scale bars are 200 μm. b, Metagene analysis of read densities at the 5′ and the 3′ regions of viral protein coding genes as measured by the different Ribo-seq approaches and RNA-seq at 7 hpi from two biological replicates. The x axis shows the nucleotide position relative to the start or the stop codons. The read densities are shown with different colours indicating the three frames relative to the main ORF (red, frame 0; black, frame +1; grey, frame +2).
