Extended Data Fig. 2: Cryo-EM data processing for cGAS–nucleosome structure.
From: Structural basis for sequestration and autoinhibition of cGAS by chromatin
a, Representative micrograph of the dataset used to determine the cGAS–nucleosome complex structure. b, Left, final reconstruction of the cGAS–nucleosome complex coloured by local resolution. Right, representation of angular distribution of particles contributing to the final map. c, Histogram and directional Fourier shell correlation (FSC) plot for the final 3D reconstitution of the cGAS–nucleosome complex (3.11 Å). A sphericity of 0.9 was determined indicating very isotropic angular distribution (a value of 1 stands for completely isotropic angular distribution). The global resolution was determined to 3.11 Å (0.143 criterion). Directional FSC determination was performed with the 3DFSC software. d, Flow chart for image processing using RELION and cryoSPARC.
