Extended Data Table 1 Cryo-EM data statistics and comparison of WT cGAS and cGAS (K394) structures

From: Structural mechanism of cGAS inhibition by the nucleosome

  1. a, Cryo-EM data collection, refinement and validation statistics; n.a., not applicable, no model was deposited. n.a.* not applicable, a local map sharpening algorithm was used (see Methods). b, Comparison of WT-cGAS-NCP and cGAS(K394E)-NCP structures. 1Map-Map correlations between WT-1cGAS-1NCP and 1cGAS(K394E)-1NCP structures, and between WT-2cGAS-2NCP and 2cGAS(K394E)-2NCP structures were calculated using low-pass filtered maps (10 Å) in combination with Chimera’s fit-in-map tool. 21cGAS(K394E)-1NCP and 2cGAS(K394E)-2NCP models were rigid-body docked into corresponding WT-cGAS-NCP maps with Chimera’s fit-in-map tool (whole molecule) and B-factor refined with Rosetta. Map-Model CC was calculated with Phenix. 3CC for cGAS chains only.
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