Extended Data Fig. 10: Microglia isolation and transplantation.
From: Microglia-organized scar-free spinal cord repair in neonatal mice
a, Representative images (left) and quantification (right) of isolated microglia (P2Y12+) from neonatal or adult Cx3cr1GFP mice (3 h after isolation, n = 400 cells examined over three independent experiments). Data are mean ± s.e.m. Scale bar, 50 μm. b, Representative images of spinal cord sections showing the activation of transplanted microglia in the adult lesion at two days after grafting. Scale bar, 250 μm. All experiments shown in b were independently repeated three times with similar results. c, Representative images of spinal cord sections showing the GFAP and CSPG staining in the adult lesion at 14 days after grafting. Quantification results are shown in Fig. 5d. Scale bar, 250 μm.
