Extended Data Fig. 7: Flowcharts of focused reconstructions for the datasets with partially homologous 67-bp dsDNA, non-homologous 120-bp dsDNA, D-loop^DG and D-loop^DH.

a–d, Data processing of partially homologous 67-bp dsDNA reaction (a), non-homologous 120-bp dsDNA reaction (b), 9-RecA–D-loop^DG complex (c) and 9-RecA–D-loop^DH complex (d). The consensus reconstruction map and the two focused refinement maps for each dataset are coloured by local resolution estimated with the RELION3 postprocess program. The resolution range is mapped to the colours in the inset next to each map. Bottom, the graphs show gold-standard FSC plots of the consensus reconstruction (blue) and two focused refinement maps (green and red), as well as the FSC curves between the composite map and the refined model (black, labelled pdb), between the composite map combining the first of the two half maps of each reconstruction and the model refined against this map (dashed pink curve, labelled half1-ref), and between the composite map combining the second of the two half maps and the model refined against the first composite half map for validation (dashed grey curve, labelled half2-val). Horizontal dashed line marks the FSC cutoff of 0.143 and the vertical dashed line indicates the resolution of the REFMAC5 refinement.