Extended Data Fig. 3: Initial 3D classification of multi-duplex classes.
From: Mechanism of strand exchange from RecA–DNA synaptic and D-loop structures
a–d, Select multi-duplex combinations containing duplexC from the 120 bp dsDNA reaction were 3D classified with partial signal subtraction and masks specific for the duplex combinations as described in methods. Shown here are the reconstructions of select classes after 3D refinement. Duplexes, their connectivity, particle number and map resolution according to the gold-standard FSC procedure are shown on top of each map. Maps are coloured by the duplex tilt (5′ tilt, cyan; 3′ tilt, purple), with S2 DNA in red. Duplex positions are labelled. A cartoon showing a simplified version of the duplex pattern for each map is shown on the left of each class. Some maps are rotated to give a clear view of obscured duplexes in the top view with rotation axis indicated. The maps are organized by the number of duplexes in each classification.
