Extended Data Fig. 12: The effects of DRB on Pol I and Pol II transcription in embryos.
From: The landscape of RNA Pol II binding reveals a stepwise transition during ZGA
a, Box plots showing top 100 major ZGA and mTBE-specific gene expression (quantile normalized FPKM) in this study and previous studies10,15 and gene body Pol II enrichment (Z-score normalized, Methods; two biological replicates). Major ZGA top 100 genes were selected by ZGA genes with highest Pol II enrichment increase (late 2-cell–one-cell) on the gene bodies. P values (t-test, two-sided, top 100 ZGA genes, sample size n = 100; and mTBE-specific gene, sample size n = 481) are shown. The median is indicated by the centre lines. The bottom, top edges and whiskers represent the 25th and 75th percentiles and 1.5 times the IQR, respectively. Gene ontology results for the top 100 major ZGA and mTBE specific genes are shown (right). b, qPCR analyses of rRNA and minor ZGA gene (Zfp352 and Zscan4) expression in DMSO- and DRB-treated early 2-cell embryos (two biological replicates). Gene expression was first normalized using either the expression of Gapdh or the absolute cell numbers, and the DRB/DMSO ratios were then computed. Data are presented as mean values. Dots indicate each data point.
