Extended Data Fig. 2: Overview of VH3-53/VH3-66-encoded human NAb structures.

a, Superimposition of Vh and Vl domains of C102 with other VH3-53- or VH3-66-encoded NAbs (top) and root mean square deviation (r.m.s.d.) calculations (bottom). b, Buried surface area comparisons for the indicated Fab–RBD structures. Buried surface areas were calculated using PDBePISA⁶⁵ and a 1.4 Å probe. c, d, Heavy-chain buried surface areas of anti-SARS-CoV-2 antibodies plotted as CDRH1 (IMGT residues 27–38) plus CDRH2 (residues 56–67*) versus CDRH3 (residues 105–117) (c), and V gene segment region (residues 1–105) versus CDRH3 (residues 106*–117) (d, asterisk indicates differences from IMGT definition). Blue data points represent 501 human antibodies complexed with protein antigens. IMGT-numbered structures (resolutions of 3.5 Å or better) were downloaded from the Structural Antibody Database (SAbDab)⁷⁰ and buried surface area were calculated using PISA⁶⁵. Buried surface areas from antibody structures with identical or near-identical heavy chain sequences were averaged to give a single point on the graph.