Extended Data Fig. 8: Purification of different SARM1 mutant proteins.
From: The NAD+-mediated self-inhibition mechanism of pro-neurodegenerative SARM1
a, b, Gel filtration profiles (a) and SDS–PAGE gels (b) of different SARM1 mutant proteins used in the NADase assay in Fig. 2. c, d, Gel filtration profiles (c) and SDS–PAGE gels (d) of different SARM1ARM+SAM mutant proteins used for the microscale thermophoresis (MST) assay. The asterisk denotes the protein band of HSP70, as identified by LC–MS (liquid chromatography–mass spectrometry). e, f, Gel filtration profiles (e) and SDS–PAGE gels (f) of different SARM1 mutant proteins used for the NADase assay in Fig. 4. The SDS–PAGE gels were stained with Coomassie blue. All the experiments were independently repeated three times. Each band of the molecular marker contains about 1–2 μg protein. For gel source data, see Supplementary Fig. 1.
