Fig. 4: The spike D614G substitution affects the susceptibility of SARS-CoV-2 to neutralization by antibodies.
a, Neutralizing activities of hamster sera against D614 and G614 mNeonGreen reporter SARS-CoV-2. Sera from hamsters infected with D614 virus (n = 8) were tested for neutralizing titres against D614- and G614-reporter SARS-CoV-2, and the 1/NT50 values are plotted. Symbols represent sera from individual hamsters. b, Ratio of 1/NT50 between D614 and G614 viruses. Symbols represent sera from individual hamsters (n = 8), the centre line represents the mean, and error bars represent s.d. c, Representative neutralizing curve of serum from an individual hamster (serum 5). Symbols represent individual replicates, the solid line represents the fitted curve, and the dotted line indicates 50% viral inhibition. d, Neutralizing activities of 11 human monoclonal antibodies against D614 and G614 mNeonGreen SARS-CoV-2. Symbols represent individual monoclonal antibodies. Data represent one of two independent experiments. e, Ratio of NT50 between D614 and G614 viruses. The averages of the NT50 ratios from two independent experiments performed in duplicate are shown. Dots represent individual monoclonal antibodies, the centre line represents the mean, and the error bars represent the s.d. f, Representative neutralizing curve of mAb18. Symbols represent individual replicates, the solid line represents the fitted curve, and the dotted line indicates 50% viral inhibition.
