Extended Data Fig. 4: SARS-CoV-2 G614 is more stable than D614.
a–c, Equal amounts (105 PFU/ml) of D614 and G614 viruses were incubated in DPBS at 42 °C (a), at 37 °C(b), or 33 °C (c), respectively. At 0 h, 0.5 h, 1 h, 2 h, 4 h, and 8 h, the viruses were quantified for their infectious levels by plaque assay on Vero E6 cells. The detect limitation of plaque assay is 10 PFU/ml. The percentage of remaining infectious viruses were normalized by the average titres at 0 h. Symbols represent individual replicates, bar heights represent the mean, and error bars represent the standard deviation. P values were determined by two-tailed Mann–Whitney test (n = 9, from three independent experiments, each conducted in triplicate), and results of P < 0.05 are indicated.
