Extended Data Fig. 7: Feedback regulations between the transcription factors and auxin signalling.

a, Expression of several TFs are regulated by auxin, which proves feedback regulation from auxin signalling output primarily on ARF8 expression. Expression was measured after treatment with 1 μM IAA for 30 min, 1h or 3h⁵⁶. Green boxes indicate upregulation, blue boxes indicate downregulation of gene expression compared with a mock treatment. b, Schematic representation of ARF8 regulation with feedbacks. Feedback from auxin signalling on regulatory TFs is expected to induce complex nonlinear regulation of ARF8 expression (see also Supplementary Note 2). c, Diagrammatic representation of the interactions taking place for different instances of model analysed in Supplementary Note 2. The two diagrams on the right (without feedback) are identical. However, for comparison with the models with feedback the parameters used for these differ (see Supplementary Note 2). d–g, left, bar chart displaying concentrations before and after knock out of transcription factor X, where Y is activated (d) or repressed (f) by ARF. Right, contour plot displaying ARF transcription rate before and after knock out of transcription factor X relative to Y and X populations, where Y is activated (f) or repressed (g) by ARF. Steady-state (SS) values corresponding to the bar plot are also reported. These results are discussed in Supplementary Note 2.