Extended Data Fig. 8: Phase separation of SYD-2 and ELKS-1 is required for proper synaptic function.
From: Assembly of synaptic active zones requires phase separation of scaffold molecules
a, Left, the C. elegans egg-laying neuronal circuit44. The hermaphrodite-specific neuron (HSN) extends an axon across the vulval region, making specific synaptic contacts with vm1/2 vulval muscles and inhibitory VC4/5 motor neurons. Right, experimental setup. HSN is activated by channelrhodopsin ChR2 with blue light, while contraction of the vm2 muscle is measured with the Ca2+ sensor GCaMP5 (ref. 21). b, Representative vulval-muscle Ca2+ traces, detected by GCaMP5, in the indicated genotypes. ‘−ATR’ conditions lacked the essential ChR2 cofactor all-trans retinol. c, Quantification of the Ca2+ spike frequency, measured using GCaMP5, in the indicated genotypes. Data are means ± 95% CI from independent animals, with comparisons made using one-way ANOVA and Dunnett’s test.
