Extended Data Fig. 10: Loss of liquidity of SYD-2 and ELKS-1 impairs the scaffolding of GIT-1.
From: Assembly of synaptic active zones requires phase separation of scaffold molecules
a, Endogenous GFP–ELKS-1(LLPS–) at early (1.5-fold embryo) nerve-ring synapses. Insets show FRAP images of bleached synapses. Main scale bar, 5 μm; inset scale bars, 1 μm. b, Quantification of nerve-ring GFP–ELKS-1 FRAP dynamics. Data are means ± 95% CI across two independent experiments. Wild-type data are from Fig. 1. c, FRAP dynamics of in vitro condensates comprising SYD-2 and ELKS-1 fragments. Data are means ± 95% CI from two independent experiments. Phase-separation assays were performed in 20 mM Tris pH 7.4, 150 mM NaCl and 10% PEG 3350, with 10 μM protein. Data for the ‘<5 min’ curve are from Fig. 2. d, Diagram showing the in vitro scaffolding experiment. e, f, Left, fluorescence images of the indicated condensates. Right, linescans performed through the centre of each condensate. Scale bars, 5 μm. g, Incorporation index (Iinc) for GIT-1–Dylight633. Data are means ± 95% CI from three independent experiments, with comparisons made using one-way ANOVA and Dunnett’s test.
