Extended Data Fig. 11: Timing of assembly of the presynaptic active zone in early nerve-ring synapses.
From: Assembly of synaptic active zones requires phase separation of scaffold molecules
Localization of endogenous GFP-tagged presynaptic active-zone components at sequential stages of embryo development. Each panel shows a maximum z projection of a separate embryo. Embryos are outlined in blue. A zy resliced projection through the nerve-ring region is shown to the right of each image. Red asterisks and arrowheads indicate visible localization to the nerve ring. Most puncta visible throughout the embryo are background autofluorescence, present even in untagged embryos (bottom). The timing of the emergence of fluorescence is stereotypical and was replicated in a second experiment. Scale bars, 10 μm.
