Extended Data Fig. 3: Purified constructs for in vitro phase separation assays.
From: Assembly of synaptic active zones requires phase separation of scaffold molecules
SDS–PAGE gels, stained by Coomassie blue, of the purified constructs used for in vitro assays in the indicated figures. Many constructs display a secondary band 10 kDa below the major species, due to mCherry cleavage at the fluorophore centre (Y72)33.
