Extended Data Fig. 5: Structure–function analysis of ELKS-1 phase separation.
From: Assembly of synaptic active zones requires phase separation of scaffold molecules
a, Diagram showing the structure of ELKS-1, including disordered regions and coiled-coils predicted by the indicated algorithms37,38,42. b, Purified recombinant mCherry–ELKS-1 constructs tested in an in vitro phase-separation assay. mCherry was fused to the N terminus of each sequence. c, Left panels in each column, example time-lapse analyses of in vitro condensates comprising the indicated ELKS-1 constructs, showing the presence or absence of liquid fusion behaviour. Right panels, FRAP dynamics for each ELKS-1 condensate. Data are means ± 95% CI, for at least three condensates from independent experiments. The y-axes show percentage intensity. Phase-separation assays were performed in 20 mM Tris pH 7.4, 150 mM NaCl with 10 μM protein. FRAP assays were performed on condensates formed in crowding conditions of 10% PEG, except in the case of the blue curves, where there was no crowding agent. Green or blue curves indicate a positive result (scored LLPS+) and black curves a negative result (scored LLPS−). Scale bars, 5 μm.
