Extended Data Fig. 6: Differential cellular contractility is sufficient to drive cardiomyocyte delamination and apicobasal depolarization in the absence of Nrg–Erbb2 signalling.
From: Tension heterogeneity directs form and fate to pattern the myocardial wall
a–b, Hearts of control or CA-MYL9-mScarlet-injected 96 hpf zebrafish larvae treated with DMSO or Erbb2 inhibitor; representative mid-sagittal confocal images (a–a”), and quantification (b; a’, n = 30; a”, n = 27). c–c”, Hearts of nrg2a:mRFP;myl7:LIFEACT-GFP 72 hpf zebrafish larvae injected with CA-MYL9-BFP; representative confocal images and 3D surface-rendered images (n = 17). d–e, Hearts of nrg2a:mRFP; myl7:eGFP-Podxl 96 hpf zebrafish larvae injected with CA-MYL9-BFP; representative mid-sagittal confocal images (e–e”), and quantification (d; e’, n = 12; e’’, n = 15). f, f’, Hearts of ctrl MO-, amhc MO- or amhc MO + CA-MYL9-mScarlet-injected 80 hpf zebrafish larvae; representative confocal images (f), and quantification (f’, n = 18). g–g” Hearts of ctrl- or tnnt2a MO- (n = 26) injected 70 hpf zebrafish embryos, transplanted with myl7:MYL9-GFP (n = 22) or myl7:CA-MYL9-GFP (n = 32) blastomeres; experimental plan (g), representative mid-sagittal confocal images (g’), and quantification (g”). Data are mean ± s.d. Two-tailed Student’s t-test (f’); Two-tailed Mann–Whitney U-test (b, d); Kruskal–Wallis test (g”). n refers to the number of hearts. Asterisks, delaminating or TL cardiomyocytes. Scale bars, 50 μm. For more details on statistics and reproducibility, see Methods.
