Extended Data Fig. 8: Notch signalling suppresses the actomyosin network in CL cardiomyocytes.
From: Tension heterogeneity directs form and fate to pattern the myocardial wall
a, a’, Representative mid-sagittal confocal and skeletonized images of 70 hpf hearts of DMSO- or Notch-inhibitor-treated zebrafish embryos (a), and quantification (a’; DMSO, n = 55; LY411575, n = 34; RO4929097; n = 35). Asterisks, TL cardiomyocytes; arrowheads, Notch+ cardiomyocytes. b, b’, Representative mid-sagittal confocal images of 94 hpf hearts of zebrafish larvae injected with NICD-P2A-tdTomato and/or CA-MYL9-GFP (b), and quantification (b’; NICD, n = 23; CA-MYL9, n = 41; NICD + CA-MYL9, n = 33). c, c’, Actin localization in Notch− and Notch+ (blue dashed line) CL cardiomyocytes of 62 hpf hearts; representative confocal images (en face view) (c), and quantification (c’, n = 20). d–d”, Phalloidin localization in Notch− and Notch+ (blue dashed line) CL cardiomyocytes of 62 hpf hearts; representative confocal images (en face view) (d), and FI profiles (d’; Notch−, n = 55; Notch+, n = 56), and quantification (d”, n = 21). e–e”, p-myo localization in NICD− and NICD+ cardiomyocytes; representative confocal images (en face view) (e), and FI profiles (n = 42) (e’), and quantification (e”; n = 21). f–f”, Representative frames from FRAP of myosin in Notch− and Notch+ CL cardiomyocytes (f), and representative recovery profiles (f’, Notch−, n = 11; Notch+, n = 12), and mobile fraction values (f”, Notch−; n = 43; Notch+ n = 47). g, g’, Representative recovery profile (g, Notch−, n = 12; Notch+, n = 9) and mobile fraction values calculated from FRAP of actin in Notch− (n = 44) and Notch+ (n = 39) CL cardiomyocytes (g’). Data are mean ± s.d., except for d’ and e’ (mean ± s.e.m.). Two-tailed Student’s t-test (d”, e”, f”, g’); two-tailed Mann–Whitney U-test (c’); Kruskal–Wallis test (a’, b’). n refers to the number of hearts (a’, b’, c’, d”, e”, f’, f”, g, g’) or number of cardiomyocytes (d’, e’). Asterisks, delaminating or TL cardiomyocytes; arrowheads, CL cardiomyocytes. Scale bars, 50 μm (a, b); 20 μm (c, d, e). For more details on statistics and reproducibility, see Methods.
