Extended Data Fig. 9: Erbb2 signalling does not regulate actomyosin localization at the onset of trabeculation.
From: Tension heterogeneity directs form and fate to pattern the myocardial wall
a–a”, Hearts of 65 hpf erbb2+/− and erbb2−/− zebrafish embryos, immunostained for Alcam, and counterstained with phalloidin and DAPI; representative MIP and en face view of boxed area (a), and FI profiles (a’, erbb2+/−, n = 116; erbb2−/−, n = 111), and quantification (a”, n = 17). b, b’, Hearts of 65 hpf erbb2+/− and erbb2−/− zebrafish embryos, immunostained for p-myo and counterstained with DAPI; representative MIP and en face view of boxed area (b), and quantification (b’, erbb2+/−, n = 17; erbb2−/−, n = 18). c–c”’, Hearts of DMSO- or Erbb2 inhibitor-treated 60 hpf zebrafish embryos, immunostained for p-myo and Alcam, and counterstained with phalloidin and DAPI; representative MIP and en face view of boxed area (c), and FI profiles (c’, DMSO, n = 237; Erbb2 inhibitor, n = 166), and quantification (c”, DMSO, n = 26; Erbb2 inhibitor, n = 22; c”’, DMSO, n = 19; Erbb2 inhibitor, n = 18). Data are mean ± s.d., except for a’ and c’ (mean ± s.e.m.). Two-tailed Student’s t-test. n refers to the number of hearts (a”, b’, c”, c”’) or number of cardiomyocytes (a’, c’). Scale bars, 50 μm. For more details on statistics and reproducibility, see Methods.
