Extended Data Fig. 10: Erbb2 signalling does not regulate actomyosin localization and stability at the onset of trabeculation.
From: Tension heterogeneity directs form and fate to pattern the myocardial wall
a, a’, Representative MIP and en face view of boxed area of 62 hpf hearts of DMSO or Erbb2 inhibitor-treated zebrafish embryos (a), and quantification (a’, DMSO, n = 25; Erbb2 inhibitor, n = 27). b–b”, Representative MIP and en face view of boxed area of 62 hpf hearts of DMSO or Erbb2- inhibitor- treated zebrafish embryos (b), and FI profiles (b’, DMSO, n = 189; Erbb2 inhibitor, n = 183), and quantification (b”, DMSO, n = 22; Erbb2 inhibitor, n = 25). c–c”, FRAP of actin in CL cardiomyocytes of DMSO or Erbb2 inhibitor-treated zebrafish embryos; representative frames (c), and representative recovery profiles (c’, DMSO, n = 16; Erbb2 inhibitor, n = 17), and mobile fraction values (c”, DMSO, n = 39; Erbb2 inhibitor, n = 45). d–d”, FRAP of myosin in CL cardiomyocytes of DMSO- or Erbb2 inhibitor-treated animals; representative frames (d), and recovery profiles (d’, n = 43), and mobile fraction values (d”, n = 42). Data are mean ± s.d., except for b’ (mean ± s.e.m.). Two-tailed Mann–Whitney U-test (a’, c”, d”); two-tailed Student’s t-test (b”). n refers to the number of hearts (a’, b”, c’, c”, d’, d”) or number of cardiomyocytes (b’). Scale bars, 50 μm. For more details on statistics and reproducibility, see Methods.
