Extended Data Fig. 1: Cardiomyocytes delaminate in a stochastic fashion.
From: Tension heterogeneity directs form and fate to pattern the myocardial wall
a, Representative frames from time-lapse beating heart imaging to visualize cardiomyocyte (CM) delamination starting at 60 hpf (n = 8/8 hearts). b–c’, Representative mid-sagittal confocal images of 65 hpf (b), and 80 hpf (c) hearts, and distribution of delaminating (de) or TL cardiomyocytes along the compact layer at 65 hpf (b’, n = 31) and 80 hpf (c’, n = 20); red dashed line: position of first de or TL cardiomyocyte; blue dashed lines: relative distance between de or TL cardiomyocytes; white asterisks: TL cardiomyocytes; magenta asterisks: delaminating cardiomyocytes; arrowheads: CL cardiomyocytes. d, d’, 60 hpf heart immunostained for N-cadherin and GFP (membrane), and counterstained with DAPI; representative mid-sagittal confocal image (d), and fluorescence intensity (d’, n = 49). e, e’, Representative mid-sagittal confocal image of 67 hpf heart (e), and fluorescence intensity (e’, n = 36). f, f’, 60 hpf heart immunostained for ZO-1 and GFP (membrane), and counterstained with DAPI; representative mid-sagittal confocal image (f), and fluorescence intensity (f’, n = 33). g, g’, Representative mid-sagittal confocal image of 65 hpf heart (g), and fluorescence intensity (g’, n = 27). h–h”, 60 hpf heart immunostained for Crb2a and GFP (membrane), and counterstained with DAPI; representative mid-sagittal confocal image (h), and fluorescence intensity (h’, n = 31), and number of delaminating cardiomyocytes with apical (yellow asterisk) or junctional (white asterisk) Crb2a localization (h”, n = 49). Data are mean ± s.d. Two-tailed Wilcoxon test. n refers to the number of cardiomyocytes. Asterisks, delaminating cardiomyocytes; arrowheads, CL cardiomyocytes. Scale bars, 50 μm (b, c, d, e, f, g, h); 5 μm (a). For more details on statistics and reproducibility, see Methods.
