Extended Data Fig. 4: Differential cellular contractility is necessary for cardiomyocyte delamination.
From: Tension heterogeneity directs form and fate to pattern the myocardial wall
a–a”, Representative mid-sagittal confocal images of hearts of WT-MYL9-GFP- (n = 25), DN-MYL9-GFP- (n = 37) or CA-MYL9-GFP- (n = 34) injected 96 hpf zebrafish larvae. b–c, Hearts of mScarlet-WT-RHOA- (WT, n = 57), mScarlet-DN-RHOA- (DN, n = 37) or mScarlet-CA-RHOA- (CA, n = 31) injected 98 hpf zebrafish larvae; representative mid-sagittal confocal images (b–b”), and quantification (c). d, d’, Hearts of mCherry-CAAX- or DN-shroom3-P2A-tdtomato-injected 96 hpf zebrafish larvae; representative mid-sagittal confocal images (d), and quantification (d’, n = 27). e, Representative frames from time-lapse imaging of a beating heart (50 hpf) of a CA-MYL9-mScarlet-injected animal (n = 5/5 hearts). f, f’, Hearts of mScarlet-DN-RHOA- and CA-MYL9-GFP-injected 100 hpf zebrafish larvae; representative mid-sagittal confocal images (f), and quantification (f’, CA-MYL9, n = 32; DN-RHOA, n = 18; DN-RHOA + CA-MYL9, n = 36). g–g”, Heart of 98 hpf zebrafish larvae transplanted with control (ctrl) or tnnt2a morpholino (MO) -injected blastomeres; experimental plan (g), representative mid-sagittal confocal images (g’), and quantification (g”, n = 17). h–h”, Hearts of ctrl (n = 12) or tnnt2a (n = 27) MO-injected 75 hpf zebrafish larvae transplanted with myl7: mKate-CAAXblastomeres; experimental plan (h), representative mid-sagittal confocal images (h’), and quantification (h”). Data are mean ± s.d. Kruskal–Wallis test (c, f’), two-tailed Student’s t-test (d’, h”). n refers to the number of hearts. Asterisks, delaminating or TL cardiomyocytes; arrowheads, CL cardiomyocytes. Scale bars, 50 μm (a–a”, b–b”, d, f, g’, h’); 5 μm (e). For more details on statistics and reproducibility, see Methods.
